A box of bee colonies includes queen bee, drone and worker bees. The responsibility of queen bee is lay eggs and they can lay eggs for a lifetime after mating. Queen bee will lay eggs at male hive room which not undergo fertilization process (non-fertilized eggs) developed into a male bee (parthenogenesis), the responsibility of the male bees are breeding offspring. In addition, the queen bee will lay eggs at the worker hive and undergo fertilization process (fertilized eggs) developed into worker bees and queen bee. Worker bees responsibilities are to collect pollen, honey, nesting, feeding larvae, cleaning the environment, defending the bee and so on.